PERMEABILITY OF MICROSOMAL MEMBRANES ISOLATED FROM RAT LIVER
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چکیده
منابع مشابه
Permeability of Microsomal Membranes Isolated from Rat Liver
Water compartments, permeability, and the possible active translocation of various substances in rat liver microsomes were studied by using radioactive compounds and ultracentrifugation. The total water of the microsomal pellet, 3.4 microl/mg dry weight, is the sum of water in the extramicrosomal and intramicrosomal spaces, or 56 and 44%, respectively. Sucrose space accounts for 77% of the intr...
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Microsomal membranes from rat liver were treated with the cross-linking reagent 1,5-difluoro-2,4-dinitrobenzene (DFDNB). Experimental work showed that at a probe concentration of 0.75 mM all free phosphatidylethanolamine (PE) and phosphatidylserine (PS) were found as dinitrophenyl derivatives: 29% of PE was in monomeric form, 9% dimeric, 2% interacted with PS, and 63% cross-linked to protein. P...
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Light-scattering measurements of osmotically induced changes in the size of rat liver microsomal vesicles pre-equilibrated in a low-osmolality buffer revealed the following. (1) The increase in extravesicular osmolality by addition of glucose 6-phosphate or mannose 6-phosphate (25 mM each) caused a rapid shrinking of microsomal vesicles. After shrinkage, a rapid swelling phase (t1/2 approx. 22 ...
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Effects of six thiol reagents with different physico-chemical properties were tested on the Na-dependent Ca + transport into the rat brain microso mal membrane vesicles. The mercurials /?-chlormercuribenzoate and Mersalyl effectively inhibited 4 Ca + uptake with IC50 values in the order of 10" mol . ľ 1 in the medium. N-ethylmaleimide and its more lipophilic analog N-(4-(2-benzoxazolyl)phenyl)...
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Rat liver rough microsomes were labeled enzymatically with (125)I using lactoperoxidase and glucose oxidase. In intact microsomes only proteins exposed on the outside face of the microsomal membrane were iodinated. Low concentrations of detergent (0.049% deoxycholate) were used to allow entrance of the iodination system into the vesicles without disassembling the membranes. This led to iodinati...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1973
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.56.3.762